FREE ASCP Technologist in Microbiology Questions and Answers

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A patient's aspirated joint fluid following a recent arthroplasty was placed on a blood agar plate. Under a microscope, gram-positive cocci were found. The colonies were alpha hemolytic following a 24-hour incubation period. Which test would be most helpful in determining the infection's cause?

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Catalase might be useful in identifying the type of bacteria as either Streptococcus spp. or Staphylococcus spp.

A throat swab was obtained from an individual who appeared to have thrush. Which of the following would make it possible to distinguish Candida albicans from other Candida species?

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Using Pagano-Levin agar, distinct species of Candida can be distinguished from one another by reducing the colorless indicator 2,3,5-triphenyltetrazolium chloride.

Which antibiotic, by binding to D-alanyl D-alanine, inhibits the formation of peptidoglycans and hence inhibits the growth of bacteria?

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An antimicrobial glycopeptide is vancomycin. The way that glycopeptides work is that they bind to D-alanyl D-alanine, which stops the peptidoglycan matrix from cross-linking and so inhibits the formation of cell walls.

A woman with a bad-smelling discharge and pruritis had a vaginal swab taken. Microscopic analysis identified the presence of protozoa with motile flagella. What is the symptoms' most likely cause?

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The parasite Trichomonas vaginalis is depicted in the figure. "Trichomonas ssp." have one posterior flagellar and four anterior flagellar. Giardia and Trichomonas can be easily identified by their flagella, which are not usually linked to vaginitis.

Since hyphae exhibit _____ between the yeast cell and the hyphae or pseudohyphae, they can be identified from germ tubes.

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Because hyphae exhibit constriction or septa between the yeast cell and the hyphae or pseudohyphae, they can be identified from germ tubes.

Which sample is most likely to contain Trichinella larvae?

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Eating undercooked meat that has nematode cysts from Trichinella spp. is the cause of trichinellosis. In the small intestine, larvae are expelled from the cyst and grow into adults.

In the Laboratory Response Network for Biological Threats, sentinel laboratories play which of the following roles?

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The Centers for Disease Control and Prevention (CDC) established the Laboratory Response Network (LRN). The LRN for Biological Threats consist of National laboratories such as the CDC and U.S. Army Medical Research Institute for Infectious Diseases (USAMRIID).

A patient, age 21, exhibits symptoms of exhaustion, headache, fever, and hurting muscles. The patient visited Uganda not too long ago. The results of their blood testing are displayed below. Which substance is the cause?

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It is possible to have contracted trypanosomiasis and malaria while in Africa. It is quite simple to distinguish between them. Trypanosoma spp. are the cause of trypanosomiasis.

Which of the following describes a cutaneous contaminant that originates in the gastrointestinal tract?

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Klebsiella species. are intestinal bacteria that are gram negative. Although Klebsiella oxytoca is often located in the gut, it can enter the skin and cause an infection.

Which culture media are commonly used to cultivate different species of Mycobacterium?

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To isolate Mycobacterium spp., Lowenstein-Jensen and Middlebrook Agar are frequently utilized. Other bacterial growth is inhibited by penicillin, nalidixic acid, Congo red, and malachite green. An enhanced medium called Middlebrook media promotes the growth of Mycobacterium species.

In tissue, what staining method can be used to identify Aspergillus fumigatus?

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The staining technique commonly used to identify Aspergillus fumigatus in tissue is Grocott's silver stain. This staining method is particularly useful for visualizing fungal structures, including the hyphae and conidia of Aspergillus species, in histopathological samples.